Thermo Scientific Velos Pro LC-MS/MS System – Introduction

The Thermo Scientific Velos Pro LC-MS/MS System is an advanced liquid chromatography tandem mass spectrometry platform designed for high-sensitivity molecular analysis, structural characterization, and complex sample identification. Developed by Thermo Fisher Scientific, the Velos Pro combines a high-performance liquid chromatography system with the innovative Dual-Pressure Linear Ion Trap (LIT) mass analyzer, providing excellent sensitivity, fast scanning capability, and powerful multi-stage mass spectrometry (MSⁿ) performance.

The Velos Pro LC-MS/MS System is widely used in proteomics, metabolomics, pharmaceutical research, biotechnology, clinical research, and life science applications. Its ability to perform detailed structural analysis makes it particularly valuable for identifying proteins, peptides, metabolites, post-translational modifications, and complex biological molecules.

The core technology of the Velos Pro is its Dual-Pressure Linear Ion Trap architecture, which separates ion storage and ion detection regions into optimized pressure zones. This design improves ion handling efficiency, increases sensitivity, and enables faster MS/MS acquisition. The system supports advanced fragmentation techniques, allowing researchers to obtain comprehensive structural information from challenging samples.

A major advantage of the Thermo Scientific Velos Pro is its powerful MSⁿ capability. Unlike conventional mass spectrometers limited to MS/MS experiments, the linear ion trap design allows multiple stages of fragmentation analysis. This capability is especially useful for detailed characterization of unknown compounds, peptide sequencing, and biomolecule identification.

The Velos Pro supports multiple ionization techniques, including Electrospray Ionization (ESI), making it highly compatible with LC-based biological analysis. When coupled with high-performance liquid chromatography, the system provides excellent separation and detection of complex mixtures with high sensitivity and reproducibility.

The instrument can be integrated with Thermo Scientific LC platforms, including Easy-nLC and other UHPLC systems, creating a complete LC-MS/MS workflow for proteomics and analytical research. Advanced software platforms such as Thermo Xcalibur Software provide comprehensive instrument control, data acquisition, qualitative analysis, and quantitative workflows.

The Velos Pro LC-MS/MS System has been widely adopted in academic research institutions, pharmaceutical companies, and biotechnology laboratories for applications including protein identification, biomarker discovery, drug metabolism studies, impurity analysis, and molecular characterization.

With its combination of high sensitivity, fast scanning speed, flexible fragmentation capabilities, and reliable operation, the Thermo Scientific Velos Pro LC-MS/MS System remains a powerful analytical platform for laboratories requiring advanced mass spectrometry performance. Refurbished Velos Pro systems are also valued in the used laboratory instrument market because they provide access to professional LC-MS/MS technology at a more economical cost.

Thermo Scientific Velos Pro LC-MS/MS System Technical Specifications