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Detailed Introduction
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader is a modular, high-performance platform designed for laboratories requiring exceptional flexibility across a broad spectrum of applications, from routine absorbance assays to advanced TR-FRET and cell-based studies. It integrates up to five detection modes in a single instrument, combining the wavelength flexibility of monochromators with the high sensitivity of filter-based optics for low-light detection.
At the heart of the Varioskan LUX is its hybrid optical system. For absorbance and fluorescence intensity, it employs high-resolution double monochromators (200–1000 nm), enabling precise spectral scanning and method development without the need for physical filter changes. For high-sensitivity applications like luminescence, time-resolved fluorescence (TRF), and AlphaScreen/AlphaLISA, the system utilizes dedicated filter-based optics to maximize light capture and signal-to-noise ratios. This dual approach ensures optimal performance whether measuring DNA/RNA quantification (A260/A280), protein assays (BCA, Bradford), ELISA, or complex HTRF kinase assays.
The system is engineered to reduce hands-on time and prevent user error. Key intelligent features include:
Automatic Dynamic Range Selection: The instrument automatically adjusts the PMT gain or reading height based on each well's signal intensity, allowing accurate measurement of both low and high signals in the same run without manual optimization.
Integrated Gas & Temperature Control: An optional gas module allows precise control of O₂ and CO₂ levels (1–21% O₂, ±1% accuracy) within the measurement chamber, facilitating live-cell kinetic assays and hypoxia studies.
Modular Upgradability: The Varioskan LUX can be configured at purchase or upgraded in the field. Options include adding a dual reagent dispenser (2–5000 µL), bottom-reading capabilities, or advanced detection modes like TRF, making it a long-term investment.
|
Parameter Category |
Specification Details |
|---|---|
|
Detection Modes |
Absorbance (UV-Vis), Fluorescence Intensity (FI), Luminescence, Time-Resolved Fluorescence (TRF/HTRF), AlphaScreen/AlphaLISA |
|
Wavelength Range |
Abs/FI: 200–1000 nm; TRF: Ex 334 nm, Em 400–700 nm; Luminescence: 360–670 nm |
|
Light Source |
Xenon flash lamp (Abs, FI, TRF), LED (AlphaScreen) |
|
Plate Formats |
6-well to 1536-well (Absorbance supports up to 384-well) |
|
Read Speed |
~15 sec for a 96-well plate (fastest mode) |
|
Sensitivity |
FI: <0.4 fmol fluorescein/well (384-black); Luminescence: <7 amol ATP/well (384-white); TRF: <1 amol/well |
|
Dynamic Range |
>6 decades (FI), >7 decades (Luminescence) |
|
Absorbance Accuracy |
±1–2% at 450 nm (0–4 Abs for 96-well) |
|
Dispensing (Optional) |
Dual dispensers, 1 mL syringes, 2–5000 µL volume range |
|
Incubation |
Up to 45°C with orbital shaking |
|
Dimensions (WxDxH) |
53 cm x 64 cm x 51 cm (approx.) |
|
Control Software |
Thermo Scientific SkanIt Software (supports endpoint, kinetic, spectral scan, and well-area scan modes) |
The Varioskan LUX is widely used in drug discovery, immunology, and molecular biology. Its ability to perform spectral scanning aids in assay optimization, while its top and bottom reading capabilities support both cuvette-style assays and cell-based reporter gene studies. The system is certified "DLReady" by Promega and "HTRF-ready" by Cisbio, ensuring compatibility with standardized assay kits. When paired with SkanIt software, it provides a robust platform for both simple QC checks and complex, automated high-throughput screening workflows.
Note: Specifications may vary based on instrument configuration (e.g., with or without dispensers, gas module). Always verify the specific configuration of a used unit against the original datasheet.
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